Naslov
Five step continuous production of PHB analyzed by elementary flux modes, yield space analysis and high structured metabolic model

Autori
Lopar, Markan ; Vrana Špoljarić, Ivna ; Atlić, Aid ; Koller, Martin ; Braunegg, Gerhart ; Horvat, Predrag

Izvornik
Biochemical engineering journal (1369-703X) 79 (2013); 57-70

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cupriavidus necator; elementary modes; high-structured mathematical model; metabolic fluxes; poly(3-hydroxybutyrate); yield space analysis

Sažetak
A high structured metabolic model for PHB synthesis by Cupriavidus necator DSM 545 consisting of 43 mass balance equations related to the same number of intracellular compounds was established. The metabolic state of cells cultivated in a continuously operated five stage bioreactor cascade was analyzed by help of elementary flux modes and two-dimensional yield space. Two different C-source feeding strategies were performed. Concerning PHB and biomass yields, values of the more efficient strategy were used as the data source for elementary modes and metabolic flux calculations, respectively. Metabolic fluxes were calculated from experimental yield data using a combination of elementary modes by applying the quadratic programming approach, in which the sum of squared weighting factors was minimized. Two different metabolic situations concerning activity of glucose-6-phosphate isomerase were tested. The high structured metabolic model was validated by comparison of experimental data from 24 h batch cultivation and simulated results.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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