Naslov
Effects of iso-osmotic NaCl and mannitol on growth, proline content, and antioxidant defense in Mammillaria gracilis Pfeiff. in vitro-grown cultures

Autori
Balen, Biljana ; Tkalec, Mirta ; Peharec Štefanić, Petra ; Rogić, Tea ; Šimac, Matija ; Rončević, Sanda ; Pitarević Svedružić, Lovorka ; Krsnik-Rasol, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
SEB Valencia 2013 abstracts / Dempster, Talja - Valencia : Society of Experimental Biology, 2013

Skup
SEB Annual Meeting 2013

Mjesto i datum
Valencia, Španjolska, 03.07.2013. - 06.07.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
salinity; osmotic stress; plant tissue culture; cactus

Sažetak
Effects of iso-osmotic concentrations of NaCl and mannitol were studied in Mammillaria gracilis (Cactaceae) callus and tumour grown in vitro. In both tissues, relative growth rates were reduced under osmotic stress, which were accompanied by a decrease in both tissue water and K+ content. However, growth was inhibited to a lesser extent after exposure to NaCl, when accumulation of Na+ ions was observed. In calli, only salinity increased proline content, whereas with tumours proline accumulated after both osmotic stresses. Osmotic stresses also induced oxidative damage in both cactus tissues, although higher oxidative injury was caused by mannitol in calli and by salt in tumours. Low iso-osmotic concentrations of NaCl (75 mM) and mannitol (150 mM) increased peroxidase, ascorbate peroxidase and esterase activities, whereas elevated catalase activity was recorded only after mannitol treatment in both tissues. High osmotic stress generally decreased enzymatic activities. However, in calli, esterase activity increased in response to high salinity, whereas ascorbate peroxidase activity was enhanced after high mannitol stress. In conclusion, both in vitro grown cactus tissues were found to be sensitive to osmotic stress caused by either mannitol or NaCl, but accumulation of Na+ ions in response to salt somewhat contributed to osmotic adjustment. However, more prominent oxidative damage induced by NaCl compared to mannitol in tumour could be related to ion toxicity. The mechanisms that mediate responses to salt- and mannitol-induced osmotic stresses differed and were dependent on tissue type.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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