Naslov
Comparison of HbA1c results by two immunoturbidimetric methods

Autori
Gugo, Katarina ; Kelava, Ines ; Tandara, Leida ; Salamunić, Ilza

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Abstracts of the 2nd European Joint Congress of EFLM and UEMS "Laboratory Medicine at the Clinical Interface" and the 7th Congress of the Croatian Society for Medical Biochemistry and Laboratory medicine (CSMBLM) : u: Biochemia Medica 22 (2012) (3) : Posters, A51-a204 / - Zagreb : Croatian Society for Medical Biochemistry and Laboratory Medicine, 2012, A88-A89

Skup
European Joint Congress of EFLM and UEMS "Laboratory Medicine at the Clinical Interface" (2 ; 2012) ; 7th Congress of the Croatian Society for Medical Biochemistry and Laboratory medicine (7 ; 2012)

Mjesto i datum
Dubrovnik, Hrvatska, 10.10.2012. - 13.10.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
methods; comparison; HbA1c
(methods; comparison HbA1c)

Sažetak
Background. The measurement of HbA1c as a biomarker for long-term monitoring of glycemic control in diabetic patients demands high analytical precision. The aim was to evaluate analytical performance of immunoturbidimetric method for measurement of HbA1c (Tina-quant Hemoglobin A1c Gen. 2)on Cobas Integra 400 plus analyzer (Roche) before introduction into routine use. Methods: Within-run and day-to-day imprecision was determined using two commercial control samples (PreciNorm and PreciPath, Roche) and one patient sample with value near the target point for good glycemic control (7% ; 54 mmol/L). Method comparison was obtained with parallel measurement of patients samples (N=75) on Dimension Xpand (Siemens) analyzer (immunoturbidimetrical method traceable to DCCT) used routinely in our laboratory. Statistical analysis was performed using MedCalc 12.2.1. software. Results: Coefficients of variation for within-run imprecision ranged (N=25): PreciNorm 0.95%, PreciPath 0.82%, and patient sample 1.16%. Coefficients of variation for day-to-day imprecision ranged (N=20): PreciNorm 0.95%, PreciPath 0.54%, patient sample 0.72%. Cumulative intralaboratory imprecision for low and high commercial controls and patient sample was 1.4%, 1.0% and 1.3% respectively. The correlation coefficient (r) between two analyzers was r=0.98. The parameters of Passing-Bablok regresion showed systematic differences among analytical systems (95% CI, a=-1.343 t0-0.400 ; b=1.000to1.143) ; Systematic differences evaluated by Bland-Altman method were Cobas-Dimension (95%CI=-0.93 t0 0.28). Conclusion: Method demonstrated high reproducibility meaning that is suitable for long-term follow-up of diabetic patients. If method is changed, constant bias should be corrected for improved results agreement.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA