Naslov
Immunochemical localization of the chloride-formate exchanger SLC26A6 (CFEX, PAT-1) in rat organs

Autori
Karaica, Dean ; Breljak, Davorka ; Ljubojević, Marija ; Herak-Kramberger, Carol M ; Micek, Vedran ; Vrhovac, Ivana ; Ivković, Jana ; Burckhardt, Birgitta C ; Burckhardt, Gerhard ; Sabolić, Ivan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Arhiv za higijenu rada i toksikologiju 64(2) / Kopjar, Nevenka - Zagreb : Institut za medicinska istraživanja i medicinu rada, 2013, 345-345

Skup
1. hrvatski simpozij o transporterima SOT-1

Mjesto i datum
Zagreb, Hrvatska, 06.06.2013. - 07.06.2013

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
enterocytes; hepatocytes; immunocytochemistry; intestine; kidney; membrane transporters; pancreas; proximal tubules; sex differences; Western blotting

Sažetak
In mice, the chloride-formate exchanger (CFEX, Slc26a6), also known as the putative anion transporter-1 (PAT-1), has been localized to the brush-border membrane (BBM) of renal proximal tubule (PT) and small intestine in mice. Its localization and protein expression in rat organs is unknown. Here, an anti-CFEX polyclonal antibody (CFEX-Ab) was used to investigate in Wistar rats the cell localization and abundance of CFEX protein in 1) kidneys, liver, gastrointestinal tract, and pancreas of adult intact males, 2) kidneys of adult male and female rats, and 3) kidneys of gonadectomised rats of both sexes. The studies were performed by immunocytochemistry in tissue cryosections and by Western blotting (WB) in isolated total cell membrane. In the kidneys, CFEX-Ab exclusively stained the BBM of PT with heterogeneous intensity (S1~S2>S3). In the liver, stained was the hepatocyte canalicular membrane, while the bile ducts were CFEX-negative. In the gastrointestinal tract, the antibody strongly stained the enterocyte BBM in duodenum and jejunum (duodenumjejunum) ; ileum, cecum, and colon remained unstained. In pancreas, CFEX protein was localized to the pancreatic duct apical membrane. A CFEX-related protein band of 100 kDa was detected in the membranes isolated from the above-mentioned organs, exhibiting zonal differences in the kidneys (cortexouter stripe) and gastrointestinal tract (duodenum>jejunum). Renal expression of CFEX protein was male- dominant, downregulated by castration, and unaffected by ovariectomy. We conclude that in rats, CFEX protein is expressed in the kidneys, liver, pancreas, and intestine, but only in the kidneys its expression exhibits stimulation by androgens.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA