Naslov
First laboratory confirmation of sleeping disease virus (SDV) in Croatia

Autori
Vardić Smrzlić, Irena ; Kapetanović, Damir ; Valić, Damir ; Teskeredžić, Emin ; McLoughlin, Marian ; Fringuelli, Elena

Izvornik
Bulletin of the European Association of Fish Pathologists (0108-0288) 33 (2013), 3; 78-83

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
sleeping disease virus; rainbow trout; RT-PCR; Croatia

Sažetak
Sleeping disease virus (SDV) is a salmonid alphavirus (SAV) recognized as a serious pathogen of farmed rainbow trout in Europe. The present study reports the first laboratory confirmation of SDV at one salmonid fish farm in Croatia. Virus isolation, real-timeRT-PCR, sera testing and histo-thology were performed for SDV detection in trout tissues examined during three sampling points: March, May and June, 2011. Virus was isolated in CHSE-214 cells from the March sample point, while ten tissue samples collected in May and subjected to RRT-PCR tested positive. The Croatian viral sequence clustered together with fresh water isolates within SAV subtype 2. VN antibodies were detected in eight sera from June and no significant histopathological lesions were observed, indicating that the fish had recovered from the infection.

Izvorni jezik
Engleski

Znanstvena područja
Geologija, Biologija, Veterinarska medicina

POVEZANOST RADA