Naslov
Anatomy of Quercus x viridis Trin., an oak of unknown origin in Croatia

Autori
Guttenberger, Helmut

Izvornik
Glasnik za šumske pokuse (0352-3861) 37 (2000); 145-159

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kongresno priopcenje, znanstveni

Ključne riječi
Anatomy; Quercus; image analysis; electron microscopy; nuc-DNA-content

Sažetak
Near Zadar, Croatia, grows an oak which is different from other local oak species in the neighborhood: Q. x viridis Trin., called green oak. To clarify the origin of this tree we investigated the anatomy of wood and leaves (in particular, the hairs and epidermis cells were examined). The investigations were done with light and scaning electron microscopic (high vacuum and ESEM) techniques combined with digital image analysis. Wood anatomy results from Q. cerris, Q. ilex and Q. pubescens were compared with data from the literature. Q. cerris, Q. ilex and Q. pubescens are supposed to be the ancestors of Q. x viridis. Investigations of the leaves were done with Q. x viridis, Q. cerris and Q. ilex. Number of measurements and histochemical reactions were done with the epidermis, hair, and root-meristem cells. EDX was used to determine the elements in the hairs, and the DNA-content of the nuclei was measured using Feulgen-reaction in combination with a densitometric method. The wood of Q. x viridis, Q. cerris and Q. pubescens is ring-porous, and of Q. ilex is difuse-porous. The anatomy of Q. x viridis and Q. cerris is very similar, triangulary ray cells were seen in preparations of these trees only. Q. x viridis has five different types of hairs, the other investigated oaks only four. Q. x viridis and Q. cerris have a similar number of hairs on the adaxial leaf surfaces. The number of hairs on the abaxial surface is different, Q. x viridis has less hairs. The size of hairs of Q. x viridis and Q. cerris is similar. We found lipoids and cuticle in all hairs, only the cell wals of the hairs of Q. x viridis are slightly woody. EDX analysis shows no flint or lime. DNA measurements have shown contents of 1.2 pg for 2 C nuclei of Q. x viridis, and 1.4 pg (root-meristem) for Q. ilex. The majority of the nuclei of hair cells have a DNA content of 2 C (all investigated oaks), some of them 4 C and 8 C (Q. cerris). The nuclei of epidermis cells have DNA content up to 16 C. Result of this investigation and the origin of the green oak is discussed.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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