Pregled bibliografske jedinice broj: 63392
Morfološke promjene u odnosu na razinu ostataka klenbuterola u jetri svinja-ženki nakon njegovog kroničnog davanja u dozi u kojoj djeluje kao promotor rasta
Morfološke promjene u odnosu na razinu ostataka klenbuterola u jetri svinja-ženki nakon njegovog kroničnog davanja u dozi u kojoj djeluje kao promotor rasta // Abstract book / - (ur.).
Zagreb: IMI, 2000. str. - (predavanje, nije recenziran, sažetak, znanstveni)
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Naslov
Morfološke promjene u odnosu na razinu ostataka klenbuterola u jetri svinja-ženki nakon njegovog kroničnog davanja u dozi u kojoj djeluje kao promotor rasta
(Morphologicl alterations related to clenbuterol residual levels in the liver of female pigs after long-term treatment with a growth-promoting dose)
Autori
Gojmerac, Tihomiira ; S. Ćurić, M. Žurić, N. Mandić,
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Abstract book
/ - Zagreb : IMI, 2000
Skup
2nd Croatian Congress of Toxicology
Mjesto i datum
Pula, Hrvatska, 09.04.2000. - 12.04.2000
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Nije recenziran
Ključne riječi
klenbuterol; jetra; svinja
(clenbuterol; liver; pig)
Sažetak
ABSTRACT
Clenbuterol is a 2-adrenergic agonist with growth promoting action. Many countries have imposed total ban on the use of clenbuterol for fattening purposes in intensive breeding since its abuse may have adverse effects on animal welfare and consumer health. The aim of the study was to investigate the relationship of morphologic alterations and the level of clenbuterol in the samples of liver homogenates of cross-bred female pigs, body mass 80-100 kg after treatment with 10 g/kg body mass clenbuterol i.m. twice daily for 41 days. The concentration of clenbuterol in the liver homogenates was determined by enzyme immunoassay (ELISA) on day 1, 7, 14, 21 and 28 after treatment. For morphologic examination, 5 m liver pararfin sections were stained with hematoxylin and eosin, or by PAS method for glycogen.
The liver level of clenbuterol was found to decrease from 115.03  5.85 ng/g on day 1 to 1.93  0.16 ng/g on day 28 after treatment. The determined levels of residual clenbuterol on post-treatment day 28 exceeded maximal allowed level of 0.5 ng/g. Morphologic liver alterations on days 1 and 28 after clenbuterol treatment manifested as a mild diffusion parenchymatous degeneration of hepatocytes and mild interstital hepatitis. The darker appearance of the liver could be the result of glycogen depletion from the cytoplasm of hepatocytes. This observation is in good correlation with previous reports on enhanced glycogenolysis induced by clenbuterol.
The results suggested that residual clenbuterol could alter the functional liver activity in female pigs.
Izvorni jezik
Engleski
Znanstvena područja
Veterinarska medicina
