Naslov
Relevance of satellite DNA genomic distribution in phylogenetic analysis: a case study with root-knot nematodes of the genus Meloidogyne

Autori
Meštrović Radan, Nevenka ; Plohl, Miroslav ; Castagnone- Sereno, Philippe

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
COST 872 Exploiting genomics to understand plant- nematode interaction, Slovenia, 2008 / - , 2008, 1-1

Skup
COST 872 Exploiting genomics to understand plant- nematode interaction

Mjesto i datum
Postojna, Slovenija, 26.05.2008. - 28.05.2008

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
satellite DNA ; genome evolution ; nematodes

Sažetak
Satellite DNAs (satDNAs) are non-coding tandemly repeated sequences that reside in all heterochromatic regions of eukaryotic chromosomes. According to the library model, not only changes in nucleotide sequence, but also changes in copy number, and/or in the composition of satellite families coexisting in a genome could result in large fluctuations among the satDNA profiles found in related species. Consequently, significant difficulties may arise in establishing sequence identities at the interspecific level, and preclude the use of satDNA sequence comparisons to infer phylogenetic relationships of the species of interest. In our previous study, three related satDNA families were shown to coexist in the form of a library in parthenogenetic RKN species, whose sequence comparison had no relevance to the phylogeny of the studied taxa. In this work, we characterized the interspecies distribution of a major satDNA from M. incognita in six congeneric RKN species reproducing either by obligate mitotic or facultative meiotic parthenogenesis, which demonstrated the library organisation of this repetitive DNA family. In addition, sequence divergence of repeats proved to bear no reliable connection to known phylogenetic relationships within the genus. Based on sequence analysis and genomic distribution of satDNAs in a set of mitotic/meiotic RKN species, we further tested the hypothesis that the distribution of satDNAs (i.e. birth/elimination of variant families) could be a relevant criterion to resolve RKN evolutionary relationships. In agreement with the library model, the sole distribution of satellite families could represent a valuable marker able to infer phylogenetic relationships among related genomes. It can be concluded that the potential of satellite nucleotide sequences as phylogenetic markers should be utilized cautiously, due to the complexity of evolution of satellite repeats.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA