Naslov
Distribution of metals in cytosol and physiological status of mussels as an estimation of trace metal exposure

Autori
Strižak, Željka ; Erk, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Interdisciplinary discourse on current environmental challenges / Melato, Aupaki Michael ; Brinkmann, Markus - , 2013, 93-93

Skup
The 3rd Young Environmental Scientists Meeting

Mjesto i datum
Kraków, Poljska, 11.02.2013. - 13.02.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Mytilus galloprovincialis; citosolni metali; citosolni metaloproteini; CEA; SE-HPLC-ICP-MS
(Mytilus galloprovincialis; cytosolic metals; cytosolic metalloproteins; CEA; SE-HPLC-ICP-MS)

Sažetak
The estimation of a trace metal exposure of the native mussel populations under influence of maritime activities using integration of biochemical and modern analytical techniques is the main subject of this study. Areas with a frequent nautic transport are affected by the trace metal pollution caused by leaching of antifouling paints which can have a permanent effect on biochemical and physiological processes in the organism. The earliest effects of the contaminants can be noticed at a cellular level and serve as biomarkers with a final goal of an effective environmental monitoring. Combining different approaches, e.g. a possibility of an organism to deal with the stress and specific trace metal responses on the cellular level can give a better final estimation of trace metal effects on the organism in the realistic environmental conditions. Therefore, two main approaches are applied on the Mediterranean mussel (Mytilus galloprovincialis) as a bioindicator organism using the digestive gland as a target tissue. General stress is estimated using a general physiological biomarker Cellular Energy Allocation (CEA) as an alternative approach to the Scope for growth (SfG). Since the metal binding to various cytosolic biomolecules as ligands represents a way of protection against the toxicity, an investigation of cytosolic metal distribution in protein fractions of different molecular masses is of a great importance for defining possible biomarkers of the trace metal exposure. This is achieved by size exclusion (SE) high performance liquid chromatography (HPLC) connected to inductively coupled plasma mass spectrometry (ICP-MS). The results will make a step towards the charachterisation of cytosolic metalloproteins responsible for metal handling in mussels under the realistic environmental exposure.

Izvorni jezik
Engleski

Znanstvena područja
Geologija, Biologija

POVEZANOST RADA