Naslov
The determination of IgE in the skin and serum of patients with atopic dermatitis

Autori
Lugović, Liborija ; Jakić-Razumović Jasminka ; Lipozenčić Jasna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Allergy European Journal of Allergy and Clinical Immunology / Van Cauwenberge, P ; Kips, J ; Bachert, C - Kopenhagen : Munksgaard, 1999

Skup
Annual Meeting of the European Academy of Allergology and Clinical Immunology

Mjesto i datum
Bruxelles, Belgija, 03.07.1999. - 07.07.1999

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
atopic dermatitis; IgE; cutaneous marker; immunohistochemistry

Sažetak
The purpose: The purpose of this study was to investigate the possible role of IgE as a cutaneous marker for atopic dermatitis (AD) in comparison with a healthy control, in relation to the measurement of serum IgE in the diagnosis of AD. Some authors reported the presence of positive IgE staining within the dermis as a specific marker for AD, but other (e.g.Brujinzeel-Komen) though that the presence of IgE molecules on Langerhans cells within the epidermis was highly specific to AD. Ward (1991) concluded that the distribution of cutaneous IgE is not solely dependent on the serum IgE level. The methods : Ten skin biopsies of AD patients and five biopsies of healthy control were analysed by immunohistochemistry with anti IgE (M7071, IgE, DAKO) and Streptovidin-biotin method. In each specimen IgE surface-bound dendritic cells were determined within the epidermis and IgE surface-bound lymphocyte-like cells within the dermis. The semi-quantative measurement of IgE positive cells was used (+high, +/- moderate, - no). The serum IgE level was determined by radiososorbent test (RIST). The results: All of the 10 (100%) AD patients had positive surface-bound IgE within the dermis and the epidermis. None of the 5 (0%) healthy controls had IgE in the epidermis although 4(80%) out of 5 had IgE in the dermis. Serum IgE level was elevated in 7 (70%) out of 10 AD patients and normal in the healthy control group. Conclusion: In AD patients the detection of IgE within the epidermis was always associated with the presence of IgE within the dermis. Atopic patients were more likely to have had detectable IgE deposition within both the epidermis and the dermis than controls. OUR results support the opinion that these methods are useful in the diagnosis of atopic dermatitis.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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