Naslov
Genetic variation in Serbian populations of Trialeurodes vaporariorum inferred from COI sequence variability

Autori
Prijović M. ; Škaljac M. ; Drobnjaković T. ; Perić P. ; Marčić D. ; Žanić K. ; Međo I. ; Puizina J.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
First International Whitefly Symposium

Mjesto i datum
Kolymvari, Grčka, 20.05.2013. - 24.05.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
T. vaporariorum; populacije; genetička varijabilnost; haplotipovi
(T. vaporariorum; populations; genetic variability; haplotypes)

Sažetak
Genetic variability of Trialeurodes vaporariorum (Hemiptera: Aleyrodidae) in Serbia was analyzed using the mitochondrial COI sequence (438 bp long). Thirteen populations and ten individuals per population were collected from different locations in Serbia and analyzed. The total number of variable sites was five, one was parsimony informative and the four were singleton. A total of six haplotypes were recorded (H1-H6). The haplotype (gene) diversity (Hd) in these specimens was very low (0.138 ± 0.042) and the corresponding nucleotide diversity (Pi) was also very low (0.0003 ± 0.001). The most frequent haplotype was H1, which was identified in all 13 analyzed populations and in 116 individuals. The second frequent haplotype H2 was identified in 5 individuals, whereas haplotypes H3-H6 were unique. The Tajima’s D value (–1.7403) and Fu’s Fs statistics (–7.293) for the entire sample are both negative and indicate that there is an excess of low frequency polymorphisms in the populations, which can imply recent population expansion. In order to identify the geographic origin of the T. vaporariorum populations that colonized Serbia, we performed phylogenetic analysis of COI haplotypes in Serbian populations and other T. vaporariorum populations retrieved from the GenBank. The final COI dataset grouped into 16 haplotypes, H1-H16, of which 13 were unique. The median joining (MJ) COI network resulted in two major lineages corresponding to the haplogroup 1 (containing haplotypes previously identified in India, China, Netherlands, Argentina and Reunion) and the haplogroup 2 (haplotypes from Costa Rica, USA and Spain). Most of the Serbian haplotypes grouped within the haplogroup 1, and the single Serbian COI haplotype H3 clustered within the haplogroup 2. Although these data suggest that there could be more independent introduction events in Serbia, additional analyzes are needed in order to reach reliable conclusions.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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