Naslov
The effect of methamphetamine on ataxia and brain malondialdehyde levels in rat model of dopaminergic neurotoxicity

Autori
Šuran, Jelena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
4th Croatian Congress of Toxicology with international participation (CROTOX 2012) : meeting abstracts ; u: Arhiv za higijenu rada i toksikologiju 63 (2012) (S2) ; Poster presentations / Želježić, Davor (ur.) - Zagreb : Institut za medicinska istraživanja i medicinu rada, 2012, 00-00

Skup
4th Croatian Congress of Toxicology with international participation (CROTOX 2012)

Mjesto i datum
Primošten, Hrvatska, 02.05.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
methamphetamine; dopaminergic neurotoxicity; ataxia; malondialdehyde

Sažetak
A single, neurotoxic methamphetamine dose administered to rat represents a valuable rodent model of dopaminergic neurodegeneration whose pathogenesis is associated with lipid peroxidation in brain, especially in nigrostriatum, and is characterized by ataxia and impaired motorics. The effect of three neurotoxic doses of methamphetamine (10, 20 and 40 mg/kg b.w., s.c.) on development of ataxia and malondialdehyde (MDA) levels in striatum, frontal cortex, cortex, hippocampus, diencephalon and cerebellum of thirty Wistar rats was evaluated. Atactic phenotype was scored 24 hours after methamphetamine administration. Total MDA in brain tissue supernatants was measured using high performance liquid chromatography with UV detection. Atactic phenotype was dose dependent, being more pronounced in animals administered 40 mg/kg (9 ± 2, mean score ± SD) in comparison to animals administered 20 mg/kg (8.25 ± 0.9) and 10 mg/kg (7 ± 1.4) of methamphetamine. Methamphetamine decreased levels of MDA in most of the brain regions ; lowest level was measured 2 hours after methamphetamine dose of 40 mg/kg in striatum (12.8 ± 2.4 nmol/g of tissue) in comparison to saline-injected control (27.2 ± 2.6 nmol/g of tissue), but it increased 24 hours later (24.9 ± 4.4 nmol/g tissue). Similar trend was present after methamphetamine doses of 20 mg/kg and 10 mg/kg, however mainly without exceeding control levels significantly. These results question the role of MDA in mechanisms of methamphetamine-induced neurotoxicity, but nevertheless confirm the effectiveness of these mechanisms in rendering animal model of monoamine disruption with a pivotal role in development of new therapeutic approaches to Parkinson disease.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA