Naslov
Chemical composition of ceramides in the yeast Candida lipolytica

Autori
Rupčić, Jasminka ; Blagović, Branka ; Varljen, Jadranka ; Marić, Vladimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Sažeci / - Rijeka, 1997

Skup
XV. hrvatski skup kemičara i kemijskih inženjera

Mjesto i datum
Opatija, Hrvatska, 24.03.1997. - 26.03.1997

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Sažetak
The discovery that sphingolipid breakdown products, ceramides and sphingoid long-chain bases, affect significant cellular responses and exhibit antitumor promoter activities in various mammalian cells has stimulated interest for these molecules as potential pharmacological agents. In contrary to mammals, the knowledge of sphingolipids in yeasts is modest. In addition to that only phospholipids have been examined. The presence of ceramides was detected only in two yeasts, Saccharomyces cerevisiae and Torulopsis utilis. In view of the lack of studies on the occurrence of ceramides inyeasts, the purpose of this work is to clarify the chemical nature of ceramide isolated from the C. lipolytica yeast. Furthermore, the data on the composition of these biological active compounds, that can be applied for specific pharmaceutical needs, could serve as base for their production using selected microorganisms. The biomass was cultivated on hexadecane as carbon source. Ceramides were isolated from total lipids in exponential and stationary growth phase. The fatty acids (Fas) and long-chain bases (LCBs) obtained after acid methanolysis, were analyzed by GLC. In exponential growth phase ceramides rich on unsaturated FAs, 22:1 being a major component. In stationary growth phase more than a half of FAs were saturated Fas and the major one was 16:0. Constituent bases were 18-, 19- and 20.phytosphingosine; 19-phytosphingosine being the major base in both growth phases.

Izvorni jezik
Engleski

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