Naslov
Laccase catalysed catechol biotransformation in a microreactor with continuous aeration

Autori
Jurinjak Tušek, Ana ; Kurtanjek, Želimir ; Zelić, Bruno

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
CD OF EXTENDED ABSTRACTS / Cvjetko Bubalo, Marina ; Šalić, Anita ; Stojkovič, Gorazd ; Zelić, Bruno ; Žnidaršič Plazl, Polona - Zagreb : Fakultet kemijskog inženjerstva i tehnologije Sveučilišta u Zagrebu, 2013, 35-36

ISBN
978-953-6470-62-4

Skup
Implementation of microreactor technology in biotechnology

Mjesto i datum
Cavtat, Hrvatska, 05.05.2013. - 08.05.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Laccase; catechol; biotransformation; microreactor; continuous aeration

Sažetak
When using oxidoreductases, enzymes that need oxygen for its activity, for biotransformations in microreactor to ensure enzyme maximum activity oxygen limitation has to be overcome. Due to their possibility to oxidize the wide range of substrates laccases are becoming more and more interesting group of oxidoreductase for application in industry and for bioremediation. Laccase use molecular oxygen to oxidize a variety of aromatic and non-aromatic hydrogen donors (e.g. phenols, polyphenols, anilines) via a mechanism involving radicals. Laccase uses oxygen as the electron acceptor to remove protons from phenolic hydroxyl groups. This reaction gives rise to radicals that can spontaneously rearrange, which can lead to fission of C-C or C-O bonds of the alkyl side chains, or to cleavage of aromatic rings. In this work laccase from Trametes vericolor was used for biotransformation of catechol (1, 2-dihydroxybenzene) in a microreactor constructed with three inflow and two outflows and volume of V = 4 mm3. One inflow was used for introduction of aqueous solution of catechol in phosphate buffer and the second one for introduction aqueous suspension of laccase in phosphate buffer. To ensure sufficient amount of dissolved oxygen in the described system a third inflow was used for continuous aeration with air. The process was described with simple mathematical model where two phase microreactor was described as pseudo-homogenous reactor:

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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