Naslov
Coenzyme regeneration in microreactor catalyzed by alcohol dehydrogenase immobilized on Fe2O3 nanoparticles

Autori
Šalić, Anita ; Hojnik Podrepšek, Gordana ; Habulin, Maja ; Zelić, Bruno

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
CD OF EXTENDED ABSTRACTS / Cvjetko Bubalo, Marina ; Šalić, Anita ; Stojkovič, Gorazd ; Zelić, Bruno ; Žnidaršič Plazl, Polona - Zagreb : Fakultet kemijskog inženjerstva i tehnologije Sveučilišta u Zagrebu, 2013, 29-30

ISBN
978-953-6470-62-4

Skup
Implementation of Microreactor Technology in Biotechnology

Mjesto i datum
Cavtat, Hrvatska, 05.05.2013. - 08.05.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
NAD+ coenzyme regeneration; immobilization; ADH; nanoparticles; microreactor

Sažetak
In the last two decades, microreactor technology has seen exponential growth. Decreased channel dimensions of microreactors (on the order of 10 - 1000 μm) afford numerous advantages that lead to increased reaction efficiency. Although a great majority of the reaction systems that are studied in microreactors are connected with chemical synthesis, biocatalysis in a microreactor was shown to be a promising alternative. In order to overcome some disadvantages of suspended biocatalyst usage, like decrease of stability in time and during storage, biocatalyst immobilization is often implemented in microreactors. The immobilization methods can be classified into two categories according to the immobilization location: direct immobilization on the microchannel wall and immobilization on a solid support inside channel by using nanoporous scaffolds, microbeads or magnetic nanoparticles (MNPs). Among them, MNPs not only offer large surface area, chemical and physical stability, and harmonious size, but also can be conveniently manipulated by magnets. Alcohol dehydrogenase, which catalyzes the oxidation of alcohols and the reduction of carbonyl compounds, has attracted attention because of its potential applications in the production of various starting materials and intermediates in chemical industry, the synthesis of chiral compounds, the regeneration of coenzymes and biosensors. Unfortunately, its stability is poor and hence limits its practical application. Immobilization is one of the efficient methods to improve enzyme stability and microreactor technology is one of the efficient methods to increase productivity.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo

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