Structure–function relationships of two paralogous single-stranded DNA-binding proteins from Streptomyces coelicolor : implication of SsbB in chromosome segregation during sporulation

Paradžik, Tina; Ivić, Nives; Filić, Želimira; Manjasetty, Babbu A.; Herron, Paul; Luić, Marija; Vujaklija, Dušica

doi:10.1093/nar/gkt050

Pregled bibliografske jedinice broj: 627438

Structure–function relationships of two paralogous single-stranded DNA-binding proteins from Streptomyces coelicolor : implication of SsbB in chromosome segregation during sporulation

Paradžik, Tina; Ivić, Nives; Filić, Želimira; Manjasetty, Babbu A.; Herron, Paul; Luić, Marija; Vujaklija, Dušica

Structure–function relationships of two paralogous single-stranded DNA-binding proteins from Streptomyces coelicolor : implication of SsbB in chromosome segregation during sporulation // Nucleic acids research, 41 (2013), 6; 3659-3672 doi:10.1093/nar/gkt050 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 627438 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Structure–function relationships of two paralogous single-stranded DNA-binding proteins from Streptomyces coelicolor : implication of SsbB in chromosome segregation during sporulation

Autori
Paradžik, Tina ; Ivić, Nives ; Filić, Želimira ; Manjasetty, Babbu A. ; Herron, Paul ; Luić, Marija ; Vujaklija, Dušica

Izvornik
Nucleic acids research (0305-1048) 41 (2013), 6; 3659-3672

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
paralogous SSBs ; S. coelicolor ; chromosome segregation
(paralogous SSBs ; S.coelicolor ; chromosome segregation)

Sažetak
The linear chromosome of Streptomyces coelicolor contains two paralogous ssb genes, ssbA and ssbB. Following mutational analysis, we concluded that ssbA is essential, whereas ssbB plays a key role in chromosome segregation during sporulation. In the ssbB mutant, ~30% of spores lacked DNA. The two ssb genes were expressed differently ; in minimal medium, gene expression was prolonged for both genes and significantly upregulated for ssbB. The ssbA gene is transcribed as part of a polycistronic mRNA from two initiation sites, 163 bp and 75 bp upstream of the rpsF translational start codon. The ssbB gene is transcribed as a monocistronic mRNA, from an unusual promoter region, 73 bp upstream of the AUG codon. Distinctive DNA-binding affinities of single-stranded DNA-binding proteins monitored by tryptophan fluorescent quenching and electrophoretic mobility shift were observed. The crystal structure of SsbB at 1.7A° resolution revealed a common OB-fold, lack of the clamp-like structure conserved in SsbA and previously unpublished S-S bridges between the A/B and C/D subunits. This is the first report of the determination of paralogous singlestranded DNA-binding protein structures from the same organism. Phylogenetic analysis revealed frequent duplication of ssb genes in Actinobacteria, whereas their strong retention suggests that they are involved in important cellular functions.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA

Projekti:
MZOS-098-0982913-2877 - Temeljna molekularno-biološka istraživanja streptomiceta (Vujaklija, Dušica, MZOS ) ( CroRIS)
MZOS-098-1191344-2943 - Protein-ligand međudjelovanja na atomnoj razini (Luić, Marija, MZOS ) ( CroRIS)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Marija Luić (autor)