Pregled bibliografske jedinice broj: 626
Inhibition of butyrylcholinesterase (EC 3.1.1.7) and acetylcholinesterase (EC 3.1.1.8) by terbutaline and bambuterol
Inhibition of butyrylcholinesterase (EC 3.1.1.7) and acetylcholinesterase (EC 3.1.1.8) by terbutaline and bambuterol // Hrvatsko kemijsko društvo - Svečani sastanak hrvatskih biokemičara uz 20. obljetnicu osnutka društva, Zagreb (Sažeci postera, ISBN 953-6256-19-3) / Flögel, Mirna (ur.).
Zagreb: Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 1996. (poster, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 626 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Inhibition of butyrylcholinesterase (EC 3.1.1.7) and acetylcholinesterase (EC 3.1.1.8) by terbutaline and bambuterol
Autori
Simeon-Rudolf, Vera ; Škrinjarić-Špoljar, Mira ; Kovarik, Zrinka ; Buntić, Anđelka
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Hrvatsko kemijsko društvo - Svečani sastanak hrvatskih biokemičara uz 20. obljetnicu osnutka društva, Zagreb (Sažeci postera, ISBN 953-6256-19-3)
/ Flögel, Mirna - Zagreb : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 1996
Skup
Hrvatsko biokemijsko društvo-Svečani sastanak hrvatskih biokemičara uz 20. obljetnicu osnutka društva
Mjesto i datum
Zagreb, Hrvatska, 18.10.1996. - 19.10.1996
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
Bambuterol; terbutaline; reversible inhibition; progressive inhibition; human cholinesterase genotypes; recombinant mouse cholinesterase
Sažetak
The reversible inhibition by the bronchodilator terbutaline N-tert-butyl[2-(3, 5-dihydroxyphenyl)-2-hydroxyethyl]ammonium sulphate and the time course of inhibition by its bis-dimethylcarbamate prodrug, bambuterol, were followed by measuring the remaining enzyme activity with propionylthiocholine or acetylthiocholine as substrates. The aim of the study was to evaluate the rate of enzyme inhibition by bambuterol and the affinity of the leaving group terbutaline for three human serum butyrylcholinesterase genotypes: usual (UU), atypical (AA) and fluoride resistant (FS). The reaction was also measured on recombinantly derived mouse acetylcholinesterase (AChE w.t.) and mouse butyrylcholinesterase (BChE w.t.). Bambuterol inhibited the UU human BChE 70 times faster than the AA genotype. Also terbutaline had 13 times higher affinity for the UU than for the AA enzyme. Terbutaline had a 10 times higher affinity for the recombinant mouse BChE than for AChE. However, bambuterol inhibited mouse BChE about 15 000 times faster than AChE thus being among the most powerful specific inhibitors distinguishing BChE from AChE.
Izvorni jezik
Engleski
Znanstvena područja
Kliničke medicinske znanosti
POVEZANOST RADA
Projekti:
00220104
Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb
