Naslov
MOLECULAR IDENTIFICATION OF ISOLATES OF Lentinula edodes USING MICROSATELLITE MARKERS

Autori
Della Rosa, Veronica ; Reverberi, Massimo ; Fabbri, Anna A. ; Zjalic, Slaven ; Fanelli, Corrado ; Urbanelli, Sandra

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
7th European Conference on Fungal Genetics . Book of abstracts / - , 2004, 222-222

Skup
7th European Conference on Fungal Genetics

Mjesto i datum
Kopenhagen, Danska, 17.04.2004. - 20.04.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Lentinula edodes; microsatellite

Sažetak
The molecular identification of six isolates of the edible mushroom L. edodes was carried out using microsatellite markers. Microsatellite loci have recently become increasingly important as markers in the human, animal and plant genomes. These loci are highly mutable and thus are able to differentiate between related taxa, even at the level of individual isolates in a single species. They are specie-specific and so their isolation must be conducted on each new specimen through the screening of a microsatellites-enriched genomic library. Only a few single-locus microsatellite markers have been isolated in Ascomycetes and Basidiomycetes. This can be due to the structure of Eumycota’s genome, which contains only limited amounts of microsatellites. Infact their genome rarely contain more than 5% of repetitive DNA (Wostemeyer J and Kreibich 2002). The screening of the L. edodes enriched-genomic library led to the isolation of five loci containing tandem repeats, that allow to discriminate two of the six isolates analysed. The difficulty in the finding of these microsatellites confirms the high reduction in the fungal genome of the non coding sequences.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA