Pregled bibliografske jedinice broj: 625314
Characterization of lipoxygenase genes and oxylipin profile in A. ochraceus.
Characterization of lipoxygenase genes and oxylipin profile in A. ochraceus. // Journal of Plant Pathology
Perugia, Italija: Edizioni ETS, 2007. str. S56-S56 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 625314 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Characterization of lipoxygenase genes and oxylipin profile in A. ochraceus.
Autori
Punelli, Federico ; Reverberi, Massimo ; Velez, Helen ; Scarpari, Marzia ; Zjalic, Slaven ; Ricelli, Alessandra ; Dobson, Alan ; Fanelli, Corrado ; Fabbri, Anna Adele
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Journal of Plant Pathology
/ - : Edizioni ETS, 2007, S56-S56
Skup
XIV Congresso Nazionale della Società Italiana di Patologia Vegetale
Mjesto i datum
Perugia, Italija, 18.09.2007. - 21.09.2007
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Aspergillus ochraceus ; ochratoxin A ; lipoxygenase ; oxylipins ; antioxidants
Sažetak
In previous works the lipoxygenase (lox) profile of Aspergillus ochraceus (wt, OTA producer), was characterized. A lox fragment of 673 bp - DQ087531 (47-78% homology with other fungal lipoxygenase sequences), was found and studied. This fragment was used to generate a D lox mutant which showed delayed conidia formation and increase of sclerotia production. In addition, low lipoperoxide formation in the mycelium, reduced lipoxygenase activity and strong reduction of OTA biosynthesis were observed in the mutant in comparison with wt. The use of lipoxygenase inhibitors (SHAM, salicylhydroxamic acid and resveratrol) allowed to further study the role played by lipoperoxides alteration in OTA synthesis by A. ochraceus. Resveratrol showed antioxidant properties and inhibition effect on lipoxygenase and cicloxygenase in humans. In A. ochraceus wt the use of this LOX-inhibitors led to a delay of lipoxygenase activity and oxylipin profile in comparison with D lox showing a marked reduction of lipoperoxides and OTA formation. Supplementation of culture media with several fatty acids was also investigated. The complete lox gene sequence was obtained with several techniques such as Genome Walker Library, Reverse PCR and 5'-3' RACE. In conclusion, the results obtained with both wt and D lox indicate that lipoperoxidation plays a major role in controlling OTA biosyn- thesis in A. ochraceus
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Biotehnologija
