Naslov
Quantitation of Epstein-Barr virus DNA in different groups of patients based on real-time PCR

Autori
Kozić, Sanja ; Vince, Adriana ; Iščić Beš, Janja ; Đaković Rode, Oktavija ; Židovec Lepej, Snježana ; Baća Vrakela, Ivana ; Poljak, Mario ; Bozic, Michael ; Kessler, H. Harald

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Clinical Chemistry and Laboratory Medicine / Plebani, Mario - : Walter de Gruyter, 2006

Skup
Sixth International Symposium on Molecular Diagnostics

Mjesto i datum
Graz, Austrija, 25.05.2006. - 27.05.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Epstein-Barr; Real-time; EBV DNA

Sažetak
Diagnosis of Epstein-Barr virus (EBV) infection in immunocompetent patients is mainly based on serological methods and clinical presentation. In immunocompromised patients (organ transplant recipients, HIV-positives) serological diagnostics appears to be unreliable. EBV DNA level has been found to correlate with severity of illness in acute EBV infections. Monitoring of EBV DNA load fluctuations has been shown to be useful in immunocompromised patients. Goal: to determine the EBV DNA quantitatively in EDTA whole bloo ; to compare viral load among four different groups of patients. Materials and methods:Following a manual DNA extraction protocol, real-time PCR was performed using the LightCycler EBV Quantification Kit (Roche). A total of 132 clinical samples were studied. Samples were derived from 4 different groups of patients. The first group (n=34) consisted of patients with acute EBV infection and second group (n=25) of EBV-seropositive adults. The third group (n=50) consisted of immunocompromised patients includin anti-HIV antibody positives (n=25) and solid organ transplant recipients (n=25) and the fourth group (n=23) consisted of infants without history of EBV infection. Results: In clinical specimens, EBV DNA could be detected in all patients wit acute EBV infection (group 1), in one EBV-seropositive adult (group 2), in 26 (54%) immunocompromised patients (16 anti-HIV positives and 10 organ transplant recipients), and in none of the infants without history of EBV infection. When EBV DNA levels were compared between different groups, no statistically significant differences were found. Conclusion: The LightCycler EBV Quantification Kit was found to be useful for determination and monitoring of EBV DNA levels in EDTA whole blood. Quantitation of EBV DNA may allow safe diagnosis of progressive EBV disease in immunocompromised patients at early stage.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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