Naslov
Analysis of biological macromolecules by microchip capillary electrophoresis

Autori
Perkov, Tea

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Prirodoslovno-matematički fakultet

Mjesto
Zagreb

Datum
22.03

Godina
2013

Stranica
71

Mentor
Kilár, Ferenc ; Rokov-Plavec, Jasmina

Neposredni voditelj
Makszin, Lilla

Ključne riječi
electrophoretic profile; circulating tumor cells; immunomagnetic RT-PCR assay; lipopolysaccharides; microchip CE-LIF; tumor markers

Sažetak
Capillary electrophoresis (CE) is a powerful, rapid, and effective bioanalytical method. The adaptation of capillary electrophoresis to microchips has made significant progress in enhancing the development of many CE applications. Expression profiling of tumor markers associated to circulating tumor cells from cancer patients was evaluated using the DNA 1000 and high sensitivity DNA microchips. Prior to electrophoretic analysis the patients’ blood samples had undergone immunomagnetic enrichmnet and multiplex RT-PCR. The high sensitivity DNA chip enabled detection of diagnostically valuable PCR fragments, which previously could not be detected by the DNA 1000 method, indicating its higher sensitivity and better diagnostic value. The lipopolysaccharides displaying different phenotypes from various bacteria, covalently labeled by fluorescent dye, were examined using microchip capillary electrophoresis. The S-type lipopolysaccharides displayed high variability in the length and composition of the repeating units constituting O-specific chains. Furthermore, two main groups and three subgroups in one group of electrophoretic profile patterns obtained from S- type lipopolysaccharides were noticed. The method has proven to be very useful in lipopolysaccharide analysis and, thus, it may replace the routinely used SDS-PAGE combined with silver staining method.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA