Naslov
Determination of fibrinogen in lipemic plasma samples using optical measurement technique

Autori
Radišić Biljak, Vanja ; Božičević, Sandra ; Vučić Lovrenčić, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Biochemia Medica / - , 2013

Skup
2nd EFLM-BD European Conference on Preanalytical Phase Preanalytical quality improvement – in quality we trust

Mjesto i datum
Zagreb, Hrvatska, 01.03.2013. - 02.03.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
fibrinogen; lipid interference; highspeed centrifugation; optical method

Sažetak
Background: Coagulation assays performed with photo-optical coagulometers may be affected by interfering substances, including lipids. According to the NCCLS guideline clotting parameters in lipemic specimens should ideally be measured by mechanical or electromechanical methods, which are not always available. Elimination of interference by ultracentrifugation of lipemic plasma samples may affect accuracy due to concomitant sedimentation of fibrinogen. We aimed to determine the accuracy of fibrinogen measurement in lipemic plasma samples cleared by high-speed centrifugation using an optical measurement procedure. Materials and methods: Twenty-two lipemic plasma samples with triglyceride concentration range of 6.01 to 40.18 mmol/L were used in this study. Concentration of fibrinogen was measured using modified Clauss method by two measuring principles: 1) opto-mechanical measurement, accurate for lipemic samples on Siemens BFT™II Analyzer using Siemens Multifi bren* U reagent in native lipemic plasma samples ; and 2) optical measurement on Siemens Sysmex CA-560 System using Dade Thrombin Reagent in plasma aliquots cleared of lipemia by high-speed centrifugation (10500 x g, 15 minutes). The results were compared using Passing & Bablok regression. Interference of triglycerides on bias was checked by linear regression. All results were analyzed using MedCalc 9.4.2.0 statistical software (MedCalc Software bvba, Mariakerke, Belgium). Results: Passing & Bablok regression revealed no statistically signifi cant difference between the two compared procedures (regression equation: y = -0.6447 + 1.2105 x ; Intercept A = -0.6447, 95% CI = -1.1500 to 0.0000 ; Slope B = 1.2105, 95% CI = 1.0000 to 1.4000). Cusum test for linearity showed nosignificant deviation from linearity (P > 0.10). Bias between the two methods (expressed as percentage, %) was unaffected by varying triglyceride levels (R2= 0.03030, P = 0.438). Conclusions: Plasma fi brinogen can be accurately measured using a photo-optical system in grossly lipemic plasma samples c

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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