Pregled bibliografske jedinice broj: 618559
Stable expression of chimeric heavy chain antibodies in CHO cells.
Stable expression of chimeric heavy chain antibodies in CHO cells. // Single Domain Antibodies : Methods and Protocols / Walker, J.M. (ur.).
Totowa (NJ): Humana Press, 2012. str. 287-303
CROSBI ID: 618559 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Stable expression of chimeric heavy chain antibodies in CHO cells.
(Stable expression of chimeric heavy chain antibodies in CHO cells)
Autori
Agrawal, Vishal ; Slivac, Igor ; Perret, Sylvie ; Bisson, Louis ; St-Laurent, Gilles ; Murad, Yanal ; Zhang, Jianbing ; Durocher, Yves
Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, znanstveni
Knjiga
Single Domain Antibodies : Methods and Protocols
Urednik/ci
Walker, J.M.
Izdavač
Humana Press
Grad
Totowa (NJ)
Godina
2012
Raspon stranica
287-303
ISBN
978-1-61779-968-6
Ključne riječi
Fc fusions, PEImax, Antibody production, Cell line development, Antibiotic selection
Sažetak
Camelid single domain antibodies fused to noncamelid Fc regions, also called chimeric heavy chain antibodies (cHCAb), offer great potential as therapeutic and diagnostic candidates due to their relatively small size (80 kDa) and intact Fc. In this chapter, we describe two approaches, limiting dilution and minipools, for generating nonamplified Chinese hamster ovary cell lines stably expressing cHCAb in suspension and serum-free cultures using a stringent antibiotic selection. Neither of the protocols necessitates the acquisition or implementation of expensive automated infrastructures and thus could be applied in any lab with minimal cell culture setup. The given protocol allows the isolation of stable clones capable of generating up to 100 mg/L of antibody in batch mode performed in shaker flasks.
Izvorni jezik
Engleski
Znanstvena područja
Biotehnologija
