Naslov
Prevention of genotoxicity of mycotoxins ochratoxin A and fumonisin B1 by chlorophyllin

Autori
Domijan, Ana-Marija ; Gajski, Goran ; Novak, Ivana ; Gerić, Marko ; Garaj-Vrhovac, Vera

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Final Programme and Abstract Book of the Second Meeting of the Croatian Association for Cancer Research with international participation / Levanat, Sonja ; Levačić Cvok, Mirela ; Musani, Vesna ; Car, Diana ; Osmak, Maja ; Herak Bosnar, Maja ; Slade Neda ; Stojanović, Nikolina - Zagreb : Hrvatsko prirodoslovno društvo ; Institut Ruđer Bošković, 2012

Skup
Second Meeting of the Croatian Association for Cancer Research with international participation

Mjesto i datum
Zagreb, Hrvatska, 08.11.2012. - 09.11.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
mycotoxins; chlorophyllin; human lymphocytes

Sažetak
Chlorophyllin (CHL) is a semi-synthetic derivative of the natural green pigment chlorophyll shown to have beneficial properties on human health. Present study aimed to investigate its possible protective effects toward mycotoxins ochratoxin A (OTA) and fumonisin B1 (FB1), frequent contaminants of crop round the world. Both mycotoxins are implicated in tumor development in humans ; OTA in development of urinary tract tumors and FB1 in oesophageal cancer and primary liver cancer. In this study toxicological profile of CHL (0.1 to 100 µg/ml) was evaluated by measuring the cytotoxic and genotoxic (comet and micronucleus assay) potential of CHL on primary human peripheral blood lymphocytes (HPBLs). CHL, in concentration range tested, had no impact on cell viability and genome damage. In addition CHL did not induce lipid peroxidation in HPBLs and exhibited free radical scavenging activity in DPPH assay. As expected, both mycotoxins OTA (4 µM) and FB1 (20 µg/ml) produced DNA damage in HPBLs already after 1 h exposure. Possible protective role of CHL against OTA and FB1 induced cytogenotoxicity was followed by co-exposing HPBLs to CHL and OTA or FB1. Results demonstrated that CHL was able to protect against cell and DNA damage induced by OTA and FB1. In conclusion, we have shown that CHL alone is safe from the aspect of cytogenotoxicity in the concentration range tested, and could prevent cytogenotoxic potential of OTA and FB1 probably acting as an antioxidants. Thus, CHL could be a perfect candidate for exploring its possible use in anti-cancer therapy.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Javno zdravstvo i zdravstvena zaštita

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