Naslov
The MATH-BTB protein ZmMAB1 regulates spindle organization during germline development in maize

Autori
Juranić, Martina ; Srilunchang, Kanok-orn ; Krohn, Nadia ; Leljak- Levanić, Dunja ; Sprunck, Stefanie ; Dresselhaus, Thomas

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Plant Reproduction for Food / Singh, Mohan ; Bhalla, Prem ; Koltunow, Anna - Melbourne : The University of Melbourne, 2012, 97-P1-26

Skup
XXII International Congress on Sexual Plant Reproduction

Mjesto i datum
Melbourne, Australija, 13.02.2012. - 17.02.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
pollination biology and ecology; gametophyte; fertilization; imprinting; fruit; seed and stress

Sažetak
Meiosis represents a fundamental cell division process required for sexual plant reproduction in eukaryotes. Whereas in animals the product of meiosis are gametes, subsequent mitotic divisions of both male and female gametophyte development are needed for gamete formation in most plant species. Cytological studies gave an insight into gametophyte development in plants, but the knowledge about regulatory mechanisms underlying meiotic and mitotic cell cycle progression of developing gametophytes is still limited. Here, we report the identification of a maize gene encoding a protein with a MATH and a BTB domain (ZmMAB1) that displays a specific expression pattern during both male and female germline development. Silencing of ZmMAB1 leads to severe defects in chromosome segregation during meiosis I and short spindles in meiosis II causing insufficient separation and migration of nuclei as well as germline arrest. Transient expression studies of ZmMAB1 in tobacco BY-2 cells revealed a cell cycle dependent localization pattern inside and outside the nucleus. The interaction of ZmMAB1 with Cullin 3 suggests a function for ZmMAB1 as a substrate-specific adaptor protein in Cul3-based E3 ubiquitin ligase complexes, thereby providing further evidence for the importance of posttranslational regulation of both male and female germline development through the ubiquitin-proteasome pathway.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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