Naslov
Determination of combined sibship indices "gray zone" using 15 STR loci for central Bosnian human population

Autori
Musanović, Jasmin ; Filipovska-Musanović, Marijana ; Kovačević, Lejla ; Buljugić, Dženisa ; Džehverović, Mirela ; Avdić, Jasna ; Marjanović, Damir

Izvornik
Molecular biology reports (0301-4851) 39 (2012), 5; 5195-5200

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA typing; Short tandem repeat loci (STR); Cumulative/Combined sibship indices (CSI); gray zone

Sažetak
In our previous population studies of Bosnia and Herzegovina human population, we have used autosomal STR, Y-STR, and X-STR loci, as well as Y-chromosome NRY biallelic markers. All obtained results were included in Bosnian referent database. In order of future development of applied population molecular genetics researches of Bosnia and Herzegovina human population, we have examined the effectiveness of 15 STR loci system in determination of sibship by using 15 STR loci and calculating different cut-off points of combined sibship indices (CSI) and distribution of sharing alleles. From the perspective of its application, it is very difficult and complicated to establish strict CSI cut-off values for determination of the doubtless sibship. High statistically significant difference between the means of CSI values and in distribution of alleles sharing in siblings and non-siblings was noticed (P < 0.0001). After constructing the "gray zone", only one false positive result was found in three CSI cut-off levels with the highest percent of determined sibship/non-sibship at the CSI = 0.067, confirming its practical benefit. Concerning the distribution of sharing alleles, it is recommended as an informative estimator for its usage within Bosnia and Herzegovina human population.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA