Naslov
HISTOLOGICAL AND CYTOLOGICAL EXAMINATION OF RAT’ REPRODUCTIVE TISSUE AFTER SHORT-TIME INTERMITTENT RADIOFREQUENCY EXPOSURE

Autori
Trosic, Ivancica ; Matausic-Pisl, Mirjana ; Pavicic Ivan

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
7th International Workshop on Biological effects of Electromagnetic fields / - , 2013

Skup
7th International Workshop on Biological effects of Electromagnetic fields

Mjesto i datum
Valletta, Malta, 08.10.2013. - 12.10.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
radiofrequency radiation; GTEM cell; Cauda epididymis

Sažetak
The aim of experiment was to investigate the influence of radiofrequency radiation (RF) on the male rats’ testes histology and epidymal free sperm cell population. Eighteen adult male rats, (12 week-old, approximate body (weigh 350g)were divided into the two groups of nine animals. 915 MHz-radiofrequency field, power density of 2.4 W/m2 and strength of 30 V/m was generated inside the Gigahertz Transversal Electromagnetic chamber(GTEM cell). One group (n=9) was whole-body irradiated in the GTEM chamber for an hour daily during two weeks. Specific absorption rate (SAR) was calculated to be 0.6 W/kg. Sham-exposed control group of animals was included (n=9). Body weight and temperature were measured before and after each exposure treatment. Immediately after last exposure the animals were sacrificed and the testes were removed, cleaned of accessory tissue and fixed. The tissue samples were embedded in paraffin and cross-sections were stained with hematoxylin-eosin stain. Histological sections were evaluated by means of a light microscope. Cauda epididymis was flushed with warm nutrition medium. The free sperm cells were collected and their quantity, quality and morphology were microscopically determined by using a hemocytometer. No statically significant alteration in any endpoints was observed. This study found no evidence of unfavourable effect of applied 915 MHz- radiofrequency radiation on testicular function or structure.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA