Naslov
Morphometry in Preoperative Diagnosis of Pathologically Altered Parathyroid Glands

Autori
Fustar-Preradovic, Ljubica ; Đanic, Davorin ; Kardum-Skelin, Ika

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Cytopathology 23 (Supplement 1) - Abstracts of the 37th European Congress of Cytology / Herbert, Amanda - Oxford : Wiley-Blackwell, 2012, 83-83

Skup
37th European Congress of Cytology

Mjesto i datum
Cavtat, Hrvatska; Dubrovnik, Hrvatska, 30.09.2012. - 03.10.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Morphometry; preoperative diagnosis; parathyroid glands

Sažetak
Aim: Simple morphological identification of a sample as parathyroid gland tissue is not always sufficient for optimal patient treatment. Instead, patients with parathyroid gland lesions that increase the risk of disease relapse should be identified. To assess the possibility of differentiating adenoma from hyperplasia in preoperative material by use of computerized morphometric analysis in order to enable better preoperative work-up in patients with hyperparathyroidism. Methods: Samples obtained by US guided fine-needle aspiration cytology of 67 parathyroid glands without known histopathologic diagnosis were dried and stained by May-Gru¨ nwald-Giemsa. Fifty nuclei per sample were analyzed. The area, circumference, convexity, minimal and maximal radius, length and width, and factors of regularity, i.e. form factor and form factor of nucleus elongation were determined for each nucleus. Basic descriptive parameters and measures of variability (variation coefficient and standard deviation) were calculated for each continuous variable. Results: Computer analysis of cell image classified the cell nuclei into two groups. The area, circumference, minimal radius, convexity and width of the nuclei showed higher values in the hyperplasia group as compared with the adenoma group. Standard deviation also showed higher values of each of the study parameters in the former group. Conclusion: Our own observations and subjective assessment of variations in nucleus size to represent substantial characteristics differentiating parathyroid adenoma and hyperplasia in cytologic smear were quantitatively verified by the use of objective morphometric measurement and should therefore be considered valid parameters on differentiating these two entities.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA