Naslov
Investigation of influence of human hepotoietin on hepatocyte proliferation

Autori
Topić, Elizabeta ; Zadro, Renata ; Gluhak, Jelica ; Slijepčević, Milivoj

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
IX European Congress of Clinical Chemistry, Eurolab 91 / - Krakov, 1991, 177-177

Skup
IX European Congress of Clinical Chemistry

Mjesto i datum
Kraków, Poljska, 08.09.1991. - 14.09.1991

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
hepatopoietin; human hepatopoietin; hepatocyte proliferation; primary and secundary liver carcinoma; chronic hepatitis

Sažetak
From serum of liver disease patients (n=29) hepatopoietin extract was isolated with which the hepatocyte primary cultuf re was inocculated. Proliferative activity was followed by incorporation of 3H-thymidine in DNA hepatocyte. The mean of proliferative activity measured in relation to control was 149% (range 96-217%). The results of flow cytometry showed significantly higher content of DNA in phase S of cell cycle in relation to control. The mean values of DNA contents in cell phasis of inocculated hepatocytes were: %Go/G1=73+2.93, %S=20.8+4.96 i %G2+M=5.07, while the values in control were: %Go/G1=89.5+3.5, %S=10.5+2.8 i %G2+M=1.47+0.36.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA