Naslov
Method of purification human genomic DNA from whole blood

Autori
Topić, Elizabeta ; Gluhak, Jelica

Izvornik
Clinica e laboratorio (0391-2035) 15 (1991), 4; 135-138

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
isolation of human DNA; minigel electrophoresis; restriction endonucleases; DNA stability

Sažetak
The objective of this research was the isolation of for restriction appropriate DNA i.e. enough pure and intact DNA from the whole blood samples that is suitable for analysis by Southern blot i.e. analysis of restrictdion fragments. Quality assesment of isolated DNA was done by restriction endonucleases Hind III, Bam HI and Eco RI. Restriction fragments were separated by agarose gelelectrophoresis (1%) in TRIS-acetat buffer (pH 8.3). The results showed that the proposed method enables isolation of intact DNA which can be stored at -20°C during 6 months.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA