Naslov
Protein and glycoprotein patterns related to morphogenesis in horseradish tissue culture

Autori
Peharec Štefanić, Petra ; Smolko, Ana ; Horvatić, Anita ; Cindrić, Mario ; Krsnik- Rasol, Marijana ; Balen, Biljana

Izvornik
Croatica chemica acta (0011-1643) 86 (2013), 1; 27-37

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
morphogenesis ; horseradish ; glycoproteins ; lectine assay ; tissue culture ; 2-DE

Sažetak
In this study cellular proteins of in vitro grown horseradish (Armoracia lapathifolia Gilib.) tissues of different morphologies, leaves (L), teratoma (TM) and tumor (TN), were separated by one-dimensional (1-DE) and two- dimensional polyacrylamide gel electrophoresis (2-DE) in order to reveal developmentally specific proteins. Moreover, proteins were subjected to analyses by lectin assays to reveal whether the morphological differences were reflected in protein glycosylation. 1-DE glycoprotein pattern showed substantial distinctions between horseradish tissues of different morphologies, which was in contrast to silver stained protein profiles, where only slightly qualitative changes were observed. 2- DE revealed more differences in expression of cellular proteins between examined tissues in comparison to 1-DE, while 2-DE blots confirmed quantitative difference in expression of 43 kDa glycoprotein between investigated tissues. Mass spectrometry analysis identified this protein as a typical horseradish peroxidase. Obtained results imply that the oligosaccharide structures could be important determinants of specificities of horseradish tissue morphology.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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