Naslov
Application of a multi-biomarker LC-MS/MS method for assessment of human mycotoxin exposure

Autori
Warth, Benedikt ; Sulyok, Michael ; Abia, Wilfred Angie ; Šarkanj, Bojan ; Fruhmann, Philipp ; Mikula, Hannes ; Berthiller, Franz ; Schumacher, Rainer ; Hametner, Christian ; Fröhlich, Johannes ; Krska, Rudolf

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Final programme & abstracts of lectures and posters, WMF meets IUPAC 2012 / Van Egmond, Hans P. ; Krska, Rudolf ; Bastiaanse, Helena B. - Bilthoven : Bastiaanse Communication, 2012, 159-160

Skup
7th Conference of The World Mycotoxin Forum and XIIIth IUPAC International Symposium on Mycotoxins and Phycotoxins, WMF meets IUPAC

Mjesto i datum
Rotterdam, Nizozemska, 05.11.2012. - 09.11.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Biomarker; LC-MS/MS; mycotoxins; risk assessment

Sažetak
Human individuals can be exposed to a variety of mycotoxins at the same time, resulting in acute or chronic and potentially synergistic effects. Hence, there is a demand for proper exposure assessment by quantification of suitable biomarkers for different mycotoxins simultaneously. We recently developed a rapid and direct LC-MS/MS method for the quantitative measurement of fifteen mycotoxins and key metabolites in human urine including deoxynivalenol (DON), DON-3-glucuronide, DON-15-glucuronide, DOM-1, nivalenol (NIV), T-2 and HT-2 toxin, zearalenone (ZEA), ZEA-14-glucuronide, α- and β-zearalenol, fumonisins B1 and B2, ochratoxin A and aflatoxin M1. This validated method was subsequently applied in pilot studies analysing urine samples obtained from Cameroonian, Croatian and Austrian volunteers. In 63% of Cameroonian samples (n=175) at least one analyte was detected and co-exposure to different mycotoxins was frequent. In total, eleven analytes (DON, D3GlcA, D15GlcA, NIV, FB1, FB2, ZEA, alpha-ZEL, Z14GlcA, AFM1 and OTA) were determined in the samples in varying incidence and quantity. In one single sample up to six analytes (AFM1, DON, D15GlcA, NIV, FB1, and OTA) were found at the same time. In Croatian (n=40) and Austrian (n=27) samples DON metabolites were ubiquitous. The concentrations of urinary DON and its glucuronide conjugates were used to estimate the daily intake of this toxin. Notably, in Austria 33% (Warth et al., 2012. Toxicology Letters, in press) and in Croatia 80% of individuals exceeded the provisional maximum tolerated daily intake of 1 µg/kg bw/day assigned by WHO (WHO, 2001. FAO Food and Nutrition Paper Vol. 74), when assuming a urine excretion of 2 l per day, a urinary transfer rate of 72% (Turner et al., 2010. Biomarkers 15: 553-562) and a constant urinary excretion of DON throughout a day. Besides its use for exposure assessment, the method was applied to study human DON metabolism in vivo. DON and two closely eluting DON-glucuronides were determined and chromatographically separated in a naturally contaminated urine sample. In contrast to previous findings, we have tentatively identified D15GlcA as a major DON metabolite in human urine (Warth et al., 2012). These new findings clearly demonstrate the great potential of the developed multibiomarker method to critically assess exposure of humans to multiple naturally occurring mycotoxins.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

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