Naslov
Human papillomavirus in cancers of the oral cavity

Autori
Dediol, Emil ; Sabol, Ivan ; Milutin Gašperov, Nina ; Grce, Magdalena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Periodicum biologorum, Vol 114, Suppl 1 / Vitale, Branko - Zagreb : Hrvatsko prirodoslovno društvo, 2012, 71-71

Skup
HDIR-2 "From Bencj to Clinic" Second Meeting of the Croatian Association for Cancer Research with International Participation

Mjesto i datum
Zagreb, Hrvatska, 08.11.2012. - 09.11.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
HPV; cancer; oral cavity

Sažetak
Head and neck carcinoma (HNC) accounts for 3-6% of all cancer cases worldwide (600, 000 cases annually). In Croatia, annual incidence of HNC is about 1000 cases representing 4.5% of all cancer cases. HNC is associated with patients older than 40 years, males, alcoholics and heavy smokers. High-risk human papillomaviruses (HPVs) have already been recognized as an etiological factor of cervical carcinoma and anogenital cancers and are beginning to be recognized as a significant risk factor in about 26% of HNC. Several studies have shown that HPV positive tumors have better prognosis compared to HPV negative tumors. However, HNC is a diverse group of squamous cell carcinoma found in the larynx, hypopharynx, oropharynx, epipharynx, oral cavity, nose, paranasal sinuses and salivary glands and thus far HPV involvement was not convincingly proven or disproven for oral cavity malignancies. In this study we examined the prevalence of different HPV genotypes in oral cavity cancer patients with (n=77) and without (n=77) usual risk factors: heavy smoking and heavy drinking. Common HPV genotyping methods failed to resolve HPV presence and genotype in most of these formalin fixed and paraffin embedded tissues. To overcome this issue we have adapted a very short SPF broad spectrum PCR (~66bp) with the extended primers for sequencing. Of 154 samples, 27% were HPV positive. As expected, HPV16 was the most common type found (39% of positive samples) followed by HPV31 (10%). There were 14 samples (24%) with more than one HPV template of which 10 could not be resolved.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA