Naslov
Modulation of perforin expression in the decidual and peripheral blood cytotoxic lymphocytes in culture

Autori
Štrbo, Nataša ; Laškarin, Gordana ; Sotošek, Vlatka ; Randić, Ljiljana ; Podack, Eckhard R. ; Rukavina, Daniel

Izvornik
American journal of reproductive immunology (1600-0897) 42 (1999), 1; 14-21

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Perforin ; Lymphocytes ; Decidua ; Peripheral blood ; Natural killer cells
(Lymphocytes ; Decidua ; Peripheral blood ; Natural killer cells)

Sažetak
PROBLEM: Perforin (P) is a cytolytic molecule located in intracellular granules of cytotoxic lymphocytes both in the peripheral blood and decidua of pregnancy. The aim was to analyze the kinetics of P expression during in vitro culture and modulation of P expression by adherent cells, their supernatants and mitogen (PHA) stimulation. METHOD OF STUDY: P (intracellular antigen) was detected by flow cytometry in the suspension of first trimester pregnancy peripheral blood lymphocytes (PBL) and decidual lymphocytes (DL). RESULTS: A decrease of the percentage of P+ cells was obtained after 1 hr incubation and was prevented by addition of 30% of decidual adherent cells (DAC) or their supernatants. Upregulation of P expression was obtained when, in addition to adherent cells, DL and PBL were stimulated by PHA. DAC present in the culture in physiological concentrations prevent downregulation of P expression. CONCLUSION: DAC located in the vicinity of decidual cytotoxic lymphocytes, owing to their unique ability to produce a wide range of substances on demand, contribute to the high level of P expression in the decidua of pregnancy.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA