Naslov
Protein and Peptide Separations

Autori
Giacometti, Jasminka ; Josić, Djuro

Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, znanstveni

Knjiga
Liquid Chromatography, 1st Edition, Applications

Urednik/ci
Fanali, Salvatore ; Haddad, Paul R., Poole, Colin F. and Schoenmakers Peter J.

Izdavač
Elsevier

Grad
Amsterdam

Godina
2013

Raspon stranica
149-184

ISBN
9780124158061

Ključne riječi
Affinity chromatography; Chromatography on monoliths; Displacement chromatography; Hydrophobic interaction; Ion exchange; Size exclusion.

Sažetak
Since the introduction of suitable highly-porous supports and later non-porous and monolithic supports, chromatography is an indispensible method for protein separations at analytical and preparative scale. The most frequently used methods for protein chromatography are ion exchange, reversed phase, hydrophobic interaction chromatography, chromatography on hydroxyapatite and different types of affinity and pseudo-affinity chromatography. Because of the use of organic solvents during the separation in reversed phase chromatography, denaturation and loss of biological activity frequently occurs and this method is less suitable for separation of biologically active, therapeutic proteins. Chromatography on monolithic supports and chromatography in the displacement mode offer additional opportunities for fast, highly effective separation of proteins at both analytical and preparative scale.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Biotehnologija

POVEZANOST RADA