Naslov
Possible role of cellular senescence in the pathogenesis of psoriasis – expression of 53BP1, γH2AX, p16, p21 and p53 in lesional psoriatic skin

Autori
Manestar Blažić, Teo ; Peternel, Sandra ; Kaštelan, Marija ; Brajac, Ines

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
6th Croatian - Italian Symposium on Psoriasis, Abstract Book / - Rijeka, 2012

Skup
6th Croatian - Italian Symposium on Psoriasis

Mjesto i datum
Rijeka, Hrvatska, 16.11.2012. - 17.11.2012

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
psoriasis; DNA damage response; cellular senescence

Sažetak
Introduction & Objectives: Cellular senescence is a state of permanent cell cycle arrest in the G0 phase. Its importance during carcinogenesis is already recognized, while its role in other pathologies is intensively studied. Recent studies have shown that the process of cellular senescence implies increased secretion of pro-inflammatory proteins and that might be important to chronic inflammation. Entry into senescence is regulated by at least two signalling pathways, the p16/Rb-dependent pathway and the DNA damage response (DDR) pathway. The objective of this study was to analyse the expression of markers of DDR and cellular senescence in psoriatic lesions and discuss the possible role of cellular senescence in the pathogenesis of psoriasis. Material & Methods: The study comprised 10 tissue samples of psoriatic lesions and 10 of healthy skin. Immunohistochemical and immunofluorescent staining was performed on 3μm formalin-fixed paraffin-embedded sections. The percentage of keratinocytes positive for 53BP1 foci, γH2AX, nuclear p16, p21 and p53 were determined. The unpaired t-test was used to compare the two groups. Differences with p<0.05 were considered statistically significant. Results: The number of cells positive for 53BP1foci, γH2AX, p16, p21 and p53 was significantly higher in psoriasis when compared to normal skin (p<0.05). Conclusion: The increased presence of DDR and cellular senescence markers in psoriasis could be related to the high cellular proliferation, which is one the hallmarks of psoriatic epidermis. The presence of senescent cells could be one of the mechanisms that cause and maintain chronic inflammation process in the psoriatic plaque. Further studies are needed to understand this connection.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA