Naslov
Sensitivity of acetylcholinesterase from the tissues of commercially important bivalve species warty venus Venus verrucosa (Linnaeus, 1758) and Noah’s Ark shell Arca noae (Linnaeus, 1758) to organophosphorous pesticides

Autori
Perić, Lorena ; Nerlović, Vedrana ; Ribarić, Luka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Arhiv za higijenu rada i toksikologiju 63(Suppl.2) / Nevenka, Kopjar - Zagreb : Institut za medicinska istraživanja i medicinu rada, 2012

Skup
4th Croatian Congress of Toxicology CROTOX 2012

Mjesto i datum
Primošten, Hrvatska, 02.10.2012. - 05.10.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
organophosphorous pesticides; bivalves; cholinesterase

Sažetak
Marine environment is subjected to the input of wide range of chemical compounds. Among them, organophosphorous pesticides (OP’s) that are commonly applied in agriculture could represent a considerable threat to cultivated and wildlife populations of marine non-target organisms in the near-shore coastal areas. These compounds selectively inhibit acetylcholinesterase (AChE), a serine hydrolase essential for transmission of nerve signals. Kinetic properties of AChE and its sensitivity to OP’s have been investigated in the gills and adductor tissues of two commercially important bivalve species: warty venus Venus verrucosa and Noah’s ark Arca noae that are widely distributed and harvested for human consumption along the eastern Adriatic coast. Specific inhibitors eserine and BW284C51 significantly affected AChE activity in the gills and adductor of both species, revealing the similarity to vertebrate enzyme. The highest ratio Vmax/Km indicating the enzyme catalytic efficiency was found for V. verrucosa adductor and A. noae gills (0, 024 ml min -1 mg prot-1). The lowest AChE activity was detected in the gills of V. verrucosa (≤2 nmol/min/mg prot). A. noae adductor had the highest AChE activity (≥10 nmol/min/mg prot). The exposure of both bivalve species to OP resulted in a dose - dependent inhibition of AChE. The potential for use of V. verrucosa and A. noae as indicators of exposure to neurotoxic compounds in marine environment, in particular within the areas not inhabited by other common bioindicator species such as mussels, is discussed.

Izvorni jezik
Engleski

Znanstvena područja
Geologija

POVEZANOST RADA