Naslov
Retention of relict satellite DNA sequences in Anemone (Ranunculaceae)

Autori
Besendorfer, Višnja ; Mlinarec, Jelena

Izvornik
ACTA BOTANICA CROATICA (0365-0588) 72 (2013), 1; 1-12

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Anemone; FISH; library hypothesis; satellite DNA

Sažetak
Satellite DNA is a genomic component present in virtually all eukaryotic organisms. The turnover of highly repetitive satellite DNA is an important element in genome organization and evolution in plants. Here we study the presence, physical distribution and abundance of the satellite DNA family AhTR1 in Anemone. Twenty-two Anemone accessions were analyzed by PCR to assess the presence of AhTR1, while fluorescence in situ hybridization and Southern hybridization were used to determine the abundance and genomic distribution of AhTR1. The AhTR1 repeat unit was PCR-amplified only in eight phylogenetically related European Anemone taxa of the Anemone section. FISH signal with AhTR1 probe was visible only in A. hortensis and A. pavonina, showing localization of AhTR1 in the regions of interstitial heterochromatin in both species. The absence of a FISH signal in the six other taxa as well as weak signal after Southern hybridization suggests that in these species AhTR1 family appears as relict sequences. Thus, the data presented here support the “library hypothesis” for AhTR1 satellite evolution in Anemone. Similar species-specific satellite DNA profiles in A. hortensis and A. pavonina support the treatment of A. hortensis and A. pavonina as one species, i.e. A. hortensis s.l.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA