Naslov
Expression of Pannexin gap junction family in sporadic cardiac myxoma cases

Autori
Zoran Legčević, Bešić Dino, Hiršl Lea, Ramić Snježana, Bulić Jakuš Floriana, Gošev Igor, Paić Frane

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Periodicum Biologorum, Vol 114, Suppl1, 2012 / Sonja Levant, Mirela Levačić Cvok, Vesna Musani, Diana Car, Maja Osmak, Maja Herak Bosnar, Neda Slade, Nikolina Stojanović - Zagreb, 2012, 54-54

Skup
HDIR-2 From bench to clinics Second meeting of the Croatian association for cancer research with international participation

Mjesto i datum
Zagreb, Hrvatska, 08.11.2012. - 09.11.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Cardiac myxoma; left atrium; gap junctions; Panx1; Panx2; Panx3

Sažetak
BACKGROUND: Cardiac myxoma represents the most prevalent type of primary cardiac tumors in adults with very serious consequences for morbidity and mortality of affected patients. Clinically useful biomarkers and patophysiological mechanisms responsible for their etiology and progression are still vastly unknown. The tripartite pannexin family is the most recently discovered member of gap junction proteins. The functional properties and tissue distribution of known pannexin isoforms in the settings of normal and diseased human heart is still incompletely understood. In this study we examined the expression of pannexin family members in sporadic cardiac myxoma tissue in order to gain a better understanding of this neoplastic disease and expand the currently limited repertoire of its biomarkers. METHODS: The study included 39 patients with cardiac myxoma treated with surgical excision of the lesion. All studied cases were sporadic cardiac myxomas. All lesions were located in the left atrium. Expression of Panx1, Panx2 and Panx3 was studied by immunohistochemical staining and qRT-PCR. RESULTS: All three pannexin family members were immunohistochemically visualized in majority of CM samples (66.7%). Comparison of relative mRNA quantities of pannexin members (qRT-PCR positive in all tissue samples) revealed statistically significant and positive correlation (p=0.0014 ; r=0.732) between Panx3 and Panx2. Panx3 also showed the statistically significant (p= 0.009) increase in mRNA expression compared to the control tissue. CONCLUSION: All three pannexin family members are expressed in neoplastic tissue of sporadic cardiac myxoma and may play distinct role in their pathogenesis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA