Naslov
Important, distinctive role of the RSC complex in chromatin structure remodelling at the yeast PHO promoters

Autori
Musladin, Sanja ; Korber, Philipp ; Barbarić, Slobodan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts 22nd IUBMB & 37th FEBS Congress ; The FEBS Journal Volume 279, Supplement 1, September 2012 / - : Wiley-Blackwell, 2012, 498-498

Skup
22nd IUBMB & 37th FEBS Congress

Mjesto i datum
Sevilla, Španjolska, 04.09.2012. - 09.09.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
RSC cmplex; chromatin remodelling; yeast PHO promoters

Sažetak
The massive transition of chromatin structure at the yeast PHO5 promoter from a repressed to an active, open state was demonstrated to be a prerequisite for promoter activation. We have previously shown that out of 15 non-essential chromatin-remodelling ATPases examined, chromatin remodelling at the PHO5 promoter involved the activities of SWI/SNF and Ino80, but no remodelling complex critically required for the PHO5 promoter opening has been identified yet. The RSC complex is an essential chromatin-remodelling complex in yeast and has been shown to disassemble nucleosomes in vitro. As inactivation of the RSC ATPase subunit Sth1 is lethal, we have used a temperature sensitive sth1td mutant to assess the possible effect of RSC inactivation on the PHO5 chromatin remodelling. The rate of chromatin opening and the consequent activation of PHO5 promoter upon physiological induction in phosphate-free medium were significantly delayed by Sth1 depletion and this effect was even more pronounced under weaker induction conditions. Chromatin remodelling and consequent activation of a Gal4-activated PHO5 promoter variant, induced through GAL-signalling pathway, was similarly affected by RSC inactivation showing that the observed effect was independent of the induction conditions and transactivator involved. Simultaneous inactivation of SWI/SNF and RSC complexes completely prevented remodelling of the native PHO5 promoter, showing a functional interplay of the two complexes in the remodelling process. Also, inactivation of the RSC complex in the isw1 chd1 double mutant, which by itself showed a significant delay in the kinetics of PHO5 opening, completely abolished remodelling. Altogether these results pointed out a crucial, distinctive role of the RSC complex for the remodelling process in the absence of dedicated remodellers like SWI/SNF, Isw1 and Chd1. Interestingly, inactivation of the RSC complex alone or simultaneously with either SWI/SNF or Isw1 and Chd1, had no appreciable effect on chromatin remodelling at the PHO8 and PHO84 promoters, which are coactivated with PHO5.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA