Naslov
SUBCELLULAR LOCALIZATION OF ARABIDOPSIS THALIANA BPM1 PROTEIN IN BY2 AND ONION CELLS AFTER TRANSIENT TRANSFORMATION

Autori
Bauer, Nataša ; Leljak Levanić, Dunja ; Juranić, Martina ; Jelaska, Sibila

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XVII Congress of the Federation of European Societies of Plant Biology, Book of abstracts / - Valencia, 2010, P10-020

Skup
FESPB 2010, XVII Congress of the Federation of European Societies of Plant Biology

Mjesto i datum
Valencia, Španjolska, 04.07.2010. - 09.07.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
MATH-BTB; protein localization; transient transformation; BPM1

Sažetak
Proteins with BTB/POZ and MATH domains recruit substrates for ubiquitination by largest E3 ligase Cul3 complex that play an essential role in the regulation of many biologic processes. A BTB/POZ-MATH gene family in Arabidopsis thaliana (BPM)comprises of 6 members. In these report dynamics of subcellular localization of eGFP labeled BMP1 after transient transformation of BY2 and onion cells wilbl e presented. Four hours after transformation BPM1:eGFP was localized exclusively in the cytoplasm. Within next 12 hours protein accumulates in nucleus but part of it still remains in cytoplasm. After 24 hours, protein is localized exclusively in nucleus, in speckle-type form. Two days after transformation BPM1:eGFP signal completely disappears from cells. In addition, A. thaliana plants were transformed with BPM1 gene under constitutive promoter, and although they transcribe transgene no protein was detected. The further characterization of BPM1 protein is under study and the future work will be focused on protein function.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA