Naslov
Host-tissue differences in transformation of pumpkin (Cucurbita pepo L.) by Agrobacterium rhizogenes

Autori
Katavić, Vesna ; Jelaska, Sibila ; Bakran-Petricioli, Tatjana ; David, Chantal

Izvornik
Plant cell tissue and organ culture (0167-6857) 24 (1991), 1; 35-42

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Agrobacterium rhizogenes; Cucurbita pepo L.; genetic transformation; hairy-root culture

Sažetak
Agrobacterium rhizogenes (wild-type strains 8196 and 15834) transformation of pumpkin (Cucurbita pepo L.) intact seedlings grown in vivo, and 6–8-day-old excised cotyledons cultured in axenic conditions was investigated. Transformed (hairy) roots were successfully induced only on the excised cotyledons with the strain 8196, while intact seedlings failed to form hairy roots with either of the two different bacterial strains. Axenic hairy-root cultures established on MS medium without hormones grew vigorously. Mannopine was detected in all transgenic root clones examined. The peroxidase activity in transformed roots was higher compared with normal roots. Electrophoretic analyses of soluble proteins and isoperoxidases showed substantial differences between transformed and normal pumpkin roots.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA