Naslov
Cholinesterase activity and oxidative stress in rats after paraoxon poisoning and oxime therapy

Autori
Berend, Suzana ; Lucić Vrdoljak, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 4th Croatian Congress of Toxicology (CROTOX 2012) / Arhiv za higijenu rada i toksikologiju 2012, Vol. 63, Supplement 2 / Želježić, Davor - , 2012, 57-57

Skup
4th Croatian Congress of Toxicology (CROTOX 2012)

Mjesto i datum
Primošten, Hrvatska, 02.10.2012. - 05.10.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Acetycholinesterase ; Brain ; Lipid peroxidation ; Organophosphates ; Oxime K048 ; Plasma

Sažetak
Among a large number of different types of pesticides available, organophosphates (OP) are the most frequent causes of professional, suicidal or accidental intoxications. It is generally known that OPs act as irreversible inhibitors of cholinesterases (ChE/AChE), yet there are still some open questions about their non-specific effects. Another issue concerning OP poisoning is the inability of conventional therapies to provide adequate protection. The present study was undertaken to examine the impact of possible oxidative stress on OP toxicity and the efficacy of the applied therapy. Rats were injected subcutaneously with sublethal dose of paraoxon, and treated intraperitoneally 1 min later with a combination of oxime K048 (25 % of its LD50) and atropine (10 mg kg-1). Plasma and brain samples were analysed for ChE/AChE activity and concentration of thiobarbituric reactive substances (TBARS) at four different time points (0.5 h, 1 h, 6 h and 24 h) following the treatment. It is important to stress that cholinesterase activity in both plasma and brain did not return to normal even after 24 h following the exposure to paraoxon. A combination of K048 and atropine afforded high potency in restoring the activity of ChE in plasma (50 % to 60 % up to 6 h) while an increase in AChE activity in the brain did not surpass 20 %. Although applied therapy efficiently counteracted paraoxon poisoning, determined TBARS concentrations suggested that these acute treatments resulted with free radical-mediated lipid peroxidation.

Izvorni jezik
Engleski

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