Naslov
Wave BioreactorTM adenovirus serotype 6 propagation in PER.C6TM cells: process development

Autori
Mikola, Mark ; Burden, Eric ; Jug-Dujakovic, Marija ; Pearre, Christy ; Herber, Wayne ; Amanullah, Ashraf

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
217th National Meeting of American Chemical Society / - , 2004

Skup
217th National Meeting of American Chemical Society

Mjesto i datum
Anaheim (CA), Sjedinjene Američke Države, 28.03.2004. - 01.04.2004

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Cell culture; Wave BioreactorTM; Process development
(Cell culture; Wave Bioreactor; Process development)

Sažetak
A virus propagation process has been developed using disposable Wave Bioreactors™ for production of an Adenovirus serotype 6 (Ad6) vaccine vector. Initial efforts to propagate the virus resulted in decreased viral amplification compared to similar Adenovirus serotype 5 (Ad5) vectors containing different transgenes despite typical cell growth and infection processes. In order to identify the cause of the poor productivity, the effects of the multiplicity of infection (MOI) and harvest times were first investigated in roller bottles at constant viable cell concentration. No improvement in final viral concentration was measured except a modest increase at the highest MOI. Secondly, the use of an Ad6 vector which does not contain the transgene in roller bottles resulted in 10 fold higher viral concentration compared to the transgene containing vector. Next the effect of higher viable cell concentration and medium exchange were investigated in roller bottles and up to ten fold higher final viral concentrations were obtained. In order to separate the effects of medium pH, cell concentration at infection and medium exchange, an experiment was conducted in stirred bioreactors (SBRs) with pH control that was infected at different viable cell concentrations with and without a complete medium exchange. The results indicated a dependence of both viable cell concentration at infection and medium exchange. An optimum pH range was found resulting in a 10 fold increase in final virus concentration compared to the baseline Wave Bioreactor™ process without pH control. Based on these results the Wave Bioreactor™ process was modified to better control the post infection pH above the critical level resulting in a similar 10 fold final virus concentration increase.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija

POVEZANOST RADA