Naslov
Targeting of RNA to DNA by Cas3 protein provides a novel interference mechanism in cas-CRISPR immunity.

Autori
Howard, Jamieson L. ; Delmas, Stephane P. ; Ivančić-Baće, Ivana ; Bolt, Edward L.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2010 CRISPR: Mechanims and applications / John van der Oost - Wageningen : Wageningen University, 2010, 59-59

Skup
2010 CRISPR: Mechanims and applications

Mjesto i datum
Wageningen, Nizozemska, 21.10.2010. - 22.10.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
CRISPR; cas3; R-loop

Sažetak
Microbial cas-CRISPR systems give immunity against invading nucleic acids that is hypothesised to operate through an RNA interference (RNAi) mechanism. Central to this is trageting of invading DNA or RNA by host CRISPR RNA, but how this is achieved is not known. We have identified that Cas3 catalyses targeting of RNA to DNA in pairing reactions that form bona fide R-loops. Targeting requires a functional HD-motif that is conserved in Cas3 proteins, and involves nicking of DNA and RNA. We found no evidence for helicase or translocase scanning by Cas3, but an ATP-induced conformational change in Cas3 can control RNA-DNA targeting reactions. RNA-DNA pairing by Cas3 explains its requirement for phage resistance in E. coli and might be useful for development as biotechnological tool more generally.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA