Naslov
Cytosolic protein concentrations and metal distribution in acanthocephalans, as potential indicators of metal exposure, their intermediate (crustacean Gammarus fossarum) and definitive host (fish Squalius cephalus)

Autori
Filipović Marijić, Vlatka ; Dragun, Zrinka ; Krasnići, Nesrete ; Matoničkin Kepčija, Renata ; Vardić Smrzlić, Irena ; Kapetanović, Damir ; Valić, Damir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract Book, 4th Congress of the Ecologists of Macedonia with International Participation / Macedonian Ecological Society - Skopje : Macedonian Ecological Society, 2012, 144-144

ISBN
978-9989-648-24-3

Skup
4th Congress of the Ecologists of Macedonia, with International Participation

Mjesto i datum
Ohrid, Sjeverna Makedonija, 12.10.2012. - 15.10.2012

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
intestinal parasites ; amphipods ; chub ; gastrointestinal tissue ; metallothioneins ; essential/non-essential metals

Sažetak
In recent years, parasites have been recognized as potential indicators of environmental quality since they integrate the adverse exposure of various contaminants, as a species which move through the food web and are finally situated at its top. Regarding their ability to accumulate metals at concentrations that are orders of magnitude higher than those in the host tissues or the environment, fish intestinal parasites acanthocephalans are considered as potential bioindicators of metal exposure in aquatic habitats. Although the interrelation between parasites and metal contamination has gained increasing interest, so far only comparison between metal concentration in acanthocephalans and host tissues was considered, but there is no information on metal distribution or detoxification in acanthocephalans. Specific and direct response to elevated intracellular metal concentrations in the organism is represented by induction of metallothioneins, low-molecular weight proteins functioning in the regulation of essential (Cu and Zn) and detoxification of non-essential metals (Cd, Hg, Ag). In the present study we compared for the first time total cytosolic protein and metallothionein concentrations in acanthocephalans (Pomphorynchus laevis Müller, Acanthocephalus lucii Müller), their intermediate host (amphipod crustacean Gammarus fossarum Koch) and definitive host (fish Squalius cephalus L.). Preliminary data on total protein (measured spectrophotometrically at 750 nm) and metallothionein concentrations (measured by differential pulse voltammetry) indicated species-specific differences. Although acanthocephalans effectively accumulate metals, especially toxic ones, average total protein and metallothionein concentrations followed the order (mg g-1 w.w.), respectively: gastrointestinal fish tissue 75.0, 2.9 > acanthocephalans 43.5, 1.8 > amphipods 20.8, 1.4. Despite variable cytosolic protein concentrations, metal distribution among cytosolic proteins of different molecular masses, determined by SE-HPLC and HR ICP-MS, indicated metal association with specific proteins, which was comparable in all species. Association to metallothionein pool was confirmed for Cu and Cd, which are already known as metals with high binding affinity for metallothioneins.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Veterinarska medicina

POVEZANOST RADA