Naslov
Serotonin transporter in rat platelets: level of protein expression underlies differences in uptake kinetics.

Autori
Hranilović, Dubravka ; Herak-Kramberger, Carol ; Čičin-Šain, Lipa ; Sabolić, Ivan ; Jernej, Branimir

Izvornik
Life Sciences (0024-3205) 69 (2001), 1; 59-65

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
5HT transporter; platelet serotonin level; western blot; rat sublines

Sažetak
By breeding selection for the extreme values of platelet serotonin level (PSL), two sublines of Wistar-derived rats, with constitutionally high or low PSL and platelet serotonin uptake (PSU), have been developed. Searching for the basis of these differences, we performed quantitative western blot analysis of serotonin transporter (5HTt) in platelet membranes isolated from both rat sublines. A polyclonal anti-5HTt antibody labeled a single, 5HTt-related 94 kDa protein band in platelet membranes, with significantly stronger intensity in membranes from rats that exhibited a high PSL. We conclude that the inherited differences in PSL and PSU in rats, following breeding selection, are determined by the level of 5HTt expression in platelet membranes.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA