Naslov
Activity of copper, zinc-superoxide dismutase in human erythrocytes exposed to cadmium and zinc: in vitro study

Autori
Živković, Tanja ; Tariba, Blanka ; Pizent, Alica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 4th Croatian Congress of Toxicology (CROTOX 2012) / Arhiv za higijenu rada i toksikologiju 2012, Vol. 63, Supplement 2 / Želježić, Davor - , 2012

Skup
4th Croatian Congress of Toxicology with the international participation

Mjesto i datum
Primošten, Hrvatska, 02.10.2012. - 05.10.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
antioxidant enzyme; enzyme activity; simultaneous exposure; superoxide radicals; metals

Sažetak
Antioxidant enzyme copper, zinc-superoxide dismutase 1 (SOD1) is a scavenger of superoxide radicals. Since zinc acts as a co-factor responsible for its structural stability, the interaction of cadmium with zinc can affect the enzyme activity due to binding competition to the same sites. There is no data related to the influence of simultaneous exposure to cadmium and zinc on SOD1 activity. The aim of this study was to investigate such effects in human erythrocytes in vitro at doses that can be found in human body fluids. Blood erythrocytes obtained from healthy male non-smoking donor (age 24) were incubated with aqueous solution of cadmium (2 μg L exp-1) and zinc (0.5 mg L exp-1, 0.7 mg L exp-1 or 1 mg L exp-1) at 37 °C for 60 minutes. Instead of metal solutions, phosphate buffer (0.1 mol L exp-1, pH 7.0) was used in control samples (SOD1 activity was 100 %). Treatment of erythrocytes with zinc alone resulted in slight decrease, whereas treatment with cadmium alone resulted in slight increase of SOD1 activity. When erythrocytes were incubated with both cadmium and zinc, SOD1 activity initially slightly increased, but addition of 1.0 mg L exp-1 of zinc resulted in a sharp drop of SOD1 activity (remaining activity was (79±10)%. Our results have shown that simultaneous exposure to cadmium and zinc under in vitro conditions may differently affect SOD1 activity compared to each metal alone.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Javno zdravstvo i zdravstvena zaštita

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