Naslov
Influence of platinum and zinc on activity of superoxide dismutase in human erythrocytes in vitro

Autori
Tariba, Blanka ; Živković, Tanja ; Pizent, Alica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 4th Croatian Congress of Toxicology (CROTOX 2012) / Arhiv za higijenu rada i toksikologiju 2012, Vol. 63, Supplement 2 / Želježić, Davor - , 2012

Skup
4th Croatian Congress of Toxicology with the international participation

Mjesto i datum
Primošten, Hrvatska, 02.10.2012. - 05.10.2012

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
cancer treatment; inhibition of enzyme activity; reactive oxygen species; toxicity

Sažetak
Platinum-containing complexes are widely used in chemotherapy of various malignancies, although some side effects and resistance could occur. It has been indicated that reactive oxygen species may be involved in these effects. Cu, Zn-superoxide dismutase (SOD1) is an antioxidant enzyme that catalyses the dismutation of superoxide anion and protects cells from damage induced by free radicals. The aim of the study was to evaluate the in vitro effect of platinum and zinc exposure on the SOD1 activity at doses (final concentrations) that can be found in human body fluids. Aqueous solution of zinc (0.5 mg L exp-1, 0.7 mg L exp-1 or 1.0 mg L exp-1) was added to erythrocytes of a healthy, non-smoking man (age 24). After incubation for 60 min at 37 °C, aqueous solution of platinum (1.0 mg L exp-1) was added to pre-treated samples, followed by incubation for another 30 min at 37 °C. Addition of zinc to erythrocytes slightly decreased SOD1 activity, while the addition of platinum to erythrocytes pretreated with zinc resulted in a further concentration-dependent activity decrease. Inhibition of SOD1 activity was the highest when the added concentrations of zinc and platinum were both 1.0 mg L exp-1. The results indicate that the inhibitory action of platinum depends on the status of zinc in the erythrocytes. Reduction of SOD1 activity may induce a decline of the defence potency of cells against the toxicity of reactive oxygen species generated by cis-platinum used in cancer treatment, and accelerate the manifestation of the toxic effects of this drug.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Javno zdravstvo i zdravstvena zaštita

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