Naslov
Verification of the Body Fluid Application on the Sysmex XE-5000 Hematology Analyzer

Autori
Šiftar, Zoran ; Kardum Paro, Mirjana Mariana ; Nazor, Aida ; Sokolić, Ivica ; Kardum Skelin, Ika ; Flegar- Meštrić, Zlata.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Biochemia Medica 2012 ; 22(3):267-402 ; A17-A204 / Šimundić, AM - Zagreb : Hrvatsko društvo za medicinsku biokemiju i laboratorijsku medicinu (HDMBLM), 2012, A118-A118

Skup
2nd European Joint Congress of European Federation of Clinical Chemistry and Laboratory Medicine (EFCC) and European Union of Medical Specialists (UEMS)/ 7th Congress of Croatian Society of Medical Biochemistry and Laboratory Medicine (CSMBLM)

Mjesto i datum
Dubrovnik, Hrvatska, 10.10.2012. - 13.10.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
body fluid application; verification; hematology analyzer

Sažetak
Background. Aim of this study is to investigate the performance of BF application on the Sysmex XE-5000 hematology analyzer in differential diagnosis of exudates and transudates. Materials and methods. The XE-5000 BF application provides white blood cell (BF-WBC) and total nucleated cell (BF-TC), as well as erythrocyte (BF-ERC) count. BF-WBC is further subcategorized in mononuclear and polymorphonuclear cells, BF-MN and BF-PMN. Performance of application was evaluated according to CLSI H56-A guidelines. Comparison to reference methods: microscopic cell counting in Neubauer chamber and differential on MGG stained smears after citospin preparation were done. Additionally, measurement of cells on XS2100 analyzer was included in comparative study. Automated cell counting was performed in Institute of Clinical Chemistry and Laboratory Medicine, whilst the microscopic in Institute of Clinical Cytology and Cytogenetics. It was done on various fluids (pleural effusion and ascites, dialysis fluid within peritoneal dialysis treatment) specimens. For correlation calculation Passing and Bablock regression analysis is used. Results. Repeatability of BF-WBC measurements was performed on 3 concentration levels. Coefficients of variation (CVs) were 21, 14 and 7.5, and met manufacturer specifications. BF-WBC linearity was proven for measurement range of 3 to 3420. Achieved coefficients of correlation with microscopic and measured counts on Sysmex XE 2100 for 35 specimens were 0.92 and 0.98, respectively. Obtained coefficient of 0.89 was found in comparison of automated and microscopic differential on 23 samples. Conclusions. Evaluation of BF application on Sysmex XE-5000 showed significant improvement in the ability of automated hematology analyzers regarding body fluid cell count analysis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

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